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PUBLICATIONS & PATENTS

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Publications

RNA-Stabilized Coat Proteins for Sensitive and Simultaneous Imaging of Distinct Single mRNAs in Live Cells
Kuffner, C.J., Marzilli, A.M., Ngo, J.T.

Under Review.

bioxriv

SynNotch Adaptors for Regulating Gene Expression Responses to Diverse Extracellular and Matrix-Based Cues

Tran, J.C., Kuffner, C.J., Marzilli, A.M., Miller, R.E., Silfen, Z.E., McMahan, J.B., Sloas, C.D., Chen, C.S., Ngo, J.T.

Accepted at Nature Communications.

biorxiv

Fe-TAMLs as a new class of small molecule peroxidase probes for correlated light and electron microscopy

Adams, S.R., Mackey, M.R., Ramachandra, R., Deerinck, T.J., Castillon, G.A., Phan, S., Hu, J., Boassa, D., Ngo, J.T., Ellisman, M.H.

biorxiv

Controlled protein activities with viral proteases, antiviral peptides, and antiviral drugs
Tague, E.P., McMahan, J.B., Tague, N., Dunlop, M.B., Ngo, J.T.

ACS Chemical Biology 2023, 18: 1228–1236.

Tension-Tuned SynNotch Receptors for Synthetic Mechanotransduction and Intercellular Force Detection
Sloas, D.C., Tran, J.C., Marzilli, A.M., Ngo, J.T. 

Nature Biotechnology2023, 41: 1287–1295.

Feature in Nature Methods

Plasmids on AddGene

SNAP-tag and HaloTag fused proteins for HaSX8-inducible control over synthetic biological functions in engineered mammalian cells.

Dotson, H.L., Ngo, J.T. 
Under Review.

biorxiv

A Genetically Encodable and Chemically Disruptable System for Synthetic Post-Translational Modification Dependent Signaling.

McMahan, J.B., Marzilli, A.M., Tran, J.C., Kuffner, C.J., Ngo, J.T. 

In Revision.

biorxiv

High-performance multiplex drug-gated CAR circuits.

Li, H,S., Wong, N.M., Tague, E.P., Ngo, J.T., Khalil, A.S., Wong, W.W. 

Cancer Cell

biorxiv

Precision control of intrabodies in live cells. (News and Views)

Marzilli, A.M., McMahan, J.B., Ngo, J.T.

Nature Methods, 2020, 17(3):259–260.

Publisher

Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy.

Boassa, D., Lemieux, S.P., Lev-Ram, V., Hu, J., Xiong, Q., Phan, S., Mackey, M., Ramachandra, R., Peace, R.E., Adams, S.R., Ellisman, M.H., Ngo, J.T.

Cell Chemical Biology, 2019, 26(10):1407-1416.e5.  

PubMed  Publisher

Commentary in Nature Methods  

Plasmids on AddGene 

Plasmid #136601 is our reccomended option. 

Chemogenetic control of gene expression and cell signaling with antiviral drugs.

Tague, E.P.*, Dotson, H.L.*, Tunney, S.N.*, Sloas, D.C., Ngo, J.T.

Nature Methods, 2018, 15(7):519-522.  

PubMed  Publisher 
Plasmids on AddGene

Commentary in Nature Methods 
'Author File' in Nature Methods 

Coverage in Futurity

Click-electron microscopy for imaging metabolically tagged nonprotein biomolecules.

Ngo, J.T., Adams, S.R., Deerinck, T.J., Boassa, D., Rodriguez-Rivera, F., Palida, S.K., Bertozzi, C.R., Ellisman, M.H., Tsien, R.Y. 

Nature Chemical Biology, 2016, 12(6):459-65.  

PubMed  Publisher

"Greatest Hits" in Nature Chemical Biology 
Feature in Cell Chemical Biology 
Feature in Angewandte Chemie​

PKMζ, But Not PKCλ, Is Rapidly Synthesized and Degraded at the Neuronal Synapse.

Palida, S.F., Butko, M.T., Ngo, J.T., Mackey, M.R., Gross, L.A., Ellisman, M.H., Tsien, R.Y.

Journal of Neuroscience, 2015, 35(20):7736-7749.

PubMed

Cell-specific proteomic analysis in Caenorhabditis elegans.

Yuet, K.P., Doma, M.K., Ngo, J.T., Sweredoski, M.J., Graham, R.L., Moradian, A., Hess, S., Schuman, E.M., Sternberg, P.W., Tirrell, D.A.

Proc. Natl. Acad. Sci. USA. 2015, 112(9):2705-2710.

PubMed

Identification of Secreted Bacterial Proteins by Non-Canonical Amino Acid Tagging.

Mahdavi, A., Szychowski, J., Ngo, J.T., Sweredoski, M.J., Graham, R.L.J., Hess, D., Schneewind, O., Mazmanian, S.K., Tirrell, D.A.

Proc. Natl. Acad. Sci. USA. 2014, 111(1):433-438.

PubMed

Mutant Methionyl-tRNA Synthetase from Bacteria Enables Site-Selective N-terminal Labeling of Proteins Expressed in Mammalian Cells.

Ngo, J.T., Schuman, E.M., Tirrell, D.A.

Proc. Natl. Acad. Sci. USA. 2013, 110(13):4992-4997

PubMed

State-selective metabolic labeling of cellular proteins.

Ngo, J.T., Babin, B.M., Champion, J.A., Schuman, E.M., Tirrell, D.A.

ACS Chemical Biology, 2012, 7(8):1326-1330.
PubMed

Noncanonical amino acids in the interrogation of cellular protein synthesis.

Ngo, J.T., Tirrell, D.A.

Accounts of Chemical Research, 2011, 44(9):677-685.

PubMed

Cleavable biotin probes for labeling of biomolecules via azide-alkyne cycloaddition.

Szychowski, J., Mahdavi, A., Hodas, J.J., Bagert, J.D., Ngo, J.T., Landgraf, P., Dieterich D.C., Schuman, E.M., Tirrell, D.A. 

J. Am. Chem. Soc. 2010, 132(51):18351-18660.

PubMed

in situ visualization and dynamics of newly synthesized proteins in rat hippocampal neurons.

Dieterich, D.C., Hodas, J.J., Gouzer, G., Shadrin, I.Y., Ngo, J.T., Triller, A., Tirrell, D.A., Schuman, E.M. 

Nature Neuroscience, 2010, 13(7):897-905.

PubMed

Cell-selective metabolic labeling of proteins.

Ngo, J.T., Champion, J.A., Mahdavi, A., Tanrikulu, I.C., Beatty, K.E., Connor, R.E., Yoo, T.H., Dieterich, D.C., Schuman, E.M., Tirrell, D.A.

Nature Chemical Biology, 2009, 5(10):715-717.

PubMed

Unnatural amino acid incorporation into virus-like particles.

Strable, E., Prasuhn, D.E. Jr., Udit, A.K., Brown, S., Link, A.J., Ngo, J.T., Lander, G., Quispe, J., Potter, C.S., Carragher, B., Tirrell, D.A., Finn, M.G.
Bioconjugate Chemistry, 2008, 19(4):866-75.

PubMed

Commentary, etc.

Precision control of intrabodies in live cells.
Marzilli, A.M., McMahan, Ngo, J.T.

Nature Methods, 2020, 17: 159-260

Patents

SYNTHETIC NOTCH RECEPTOR PROTEIN FOR MODULATING GENE EXPRESSION

United States Patent 12065501, Granted 2024-08-20

Inventor: Ngo, John T.      

Assignee: Trustees of Boston University

https://www.freepatentsonline.com/12065501.html

COMPOSITIONS AND METHODS FOR CONTROLLED MRNA TRANSLATION AND STABILITY

United States Patent Application 20240263193, Published 2024-08-08

Inventors: Marzilli, Alexander Michael, Ngo, John Tuan

Assignee: Trustees of Boston University 

https://www.freepatentsonline.com/y2024/0263193.html

ENGINEERED EXTRACELLULAR RECEPTOR CONSTRUCTS AND USES THEREOF

United States Patent 12043652, Granted 2024-07-23

Inventors: Tague, Elliot Parker, Ngo, John T., McMahan, Jeffrey Blye

Assignee: Trustees of Boston University

https://www.freepatentsonline.com/12043652.html

CELLULAR RECEPTORS AND USES THEREOF   

United States Patent 11672828, Granted 2023-06-13

Inventors: Ngo, John T., McMahan, Jeffrey Blye

Assignee: Trustees of Boston University

https://www.freepatentsonline.com/11672828.html

NOVEL DRUG-CONTROLLED SYSTEMS AND USES THEREOF    

United States Patent Application 20220062234, Published 2022-03-03

Inventors: Ngo, John T., Dunlop, Mary, Tague, Elliot Parker, Tague, Nathan Michael, Marzilli, Alexander Michael

Assignee: Trustees of Boston University

https://www.freepatentsonline.com/y2022/0062234.html

INDUCIBLE CELL RECEPTORS FOR CELL-BASED THERAPEUTICS

United States Patent Application 20210189367, Published 2021-06-24

Inventors: Lee, Philip Janmin, Lu, Timothy Kuan-ta, Gordley, Russell Morrison, Wong, Wilson, Ngo, John          

Assignees: Senti Biosciences, Inc., Trustees of Boston University 

https://www.freepatentsonline.com/y2021/0189367.html

CHIMERIC ANTIGEN RECEPTOR (CAR) MODULATION

United States Patent 11059864, Granted 2021-07-21

Inventors: Ngo, John, Wong, Wilson, Wong, Meng Lai Nicole, Li, Huishan, Tague, Elliot P.

Assignee: Trustees of Boston University

https://www.freepatentsonline.com/11059864.html

SYNTHETIC NOTCH PROTEIN FOR MODULATING GENE EXPRESSION  

United States Patent 10858443, Granted 2020-12-08

Inventor: Ngo, John T.

Assignee: Trustees of Boston University

https://www.freepatentsonline.com/10858443.html

SYNTHETIC PROTEIN FOR MODULATING GENE EXPRESSION

United States Patent 10717782, Granted 2020-07-21

Inventor: Ngo, John T. 

Assignee: Trustees of Boston University

https://www.freepatentsonline.com/10717782.html

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