Ngo Lab 

RNA-Stabilized Coat Proteins for Sensitive and Simultaneous Imaging of Distinct Single mRNAs in Live Cells
Kuffner, C.J., Marzilli, A.M., Ngo, J.T.

Accepted, Nature Methods

bioxriv

Protease-Containing Nanobodies for Detecting and Manipulating Intracellular Antigens Using Antiviral Drugs
Le, Q. and Ngo, J.T.

ACS Chemical Biology

Fluorescein-Based SynNotch Adaptors for Regulating Gene Expression Responses to Diverse Extracellular and Matrix-Based Cues

Tran, J.C., Kuffner, C.J., Marzilli, A.M., Miller, R.E., Silfen, Z.E., McMahan, J.B., Sloas, C.D., Chen, C.S., Ngo, J.T.

Nature Communications, 2025, 16: 852

Fe-TAMLs as a new class of small molecule peroxidase probes for correlated light and electron microscopy

Adams, S.R., Mackey, M.R., Ramachandra, R., Deerinck, T.J., Castillon, G.A., Phan, S., Hu, J., Boassa, D., Ngo, J.T., Ellisman, M.H.

biorxiv

Controlled protein activities with viral proteases, antiviral peptides, and antiviral drugs
Tague, E.P., McMahan, J.B., Tague, N., Dunlop, M.B., Ngo, J.T.

ACS Chemical Biology 2023, 18: 1228–1236.

Tension-Tuned SynNotch Receptors for Synthetic Mechanotransduction and Intercellular Force Detection
Sloas, D.C., Tran, J.C., Marzilli, A.M., Ngo, J.T. 

Nature Biotechnology, 2023, 41: 1287–1295.

Feature in Nature Methods

Plasmids on AddGene

SNAP-tag and HaloTag fused proteins for HaSX8-inducible control over synthetic biological functions in engineered mammalian cells.

Dotson, H.L., Ngo, J.T. 
Under Review.

biorxiv

A Genetically Encodable and Chemically Disruptable System for Synthetic Post-Translational Modification Dependent Signaling.

McMahan, J.B., Marzilli, A.M., Tran, J.C., Kuffner, C.J., Ngo, J.T. 

In Revision.

biorxiv

High-performance multiplex drug-gated CAR circuits.

Li, H,S., Wong, N.M., Tague, E.P., Ngo, J.T., Khalil, A.S., Wong, W.W. 

Cancer Cell

biorxiv

Precision control of intrabodies in live cells. (News and Views)

Marzilli, A.M., McMahan, J.B., Ngo, J.T.

Nature Methods, 2020, 17(3):259–260.

Publisher

Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy.

Boassa, D., Lemieux, S.P., Lev-Ram, V., Hu, J., Xiong, Q., Phan, S., Mackey, M., Ramachandra, R., Peace, R.E., Adams, S.R., Ellisman, M.H., Ngo, J.T.

Cell Chemical Biology, 2019, 26(10):1407-1416.e5.  

PubMed  Publisher

Commentary in Nature Methods  

Plasmids on AddGene 

Plasmid #136601 is our reccomended option. 

Chemogenetic control of gene expression and cell signaling with antiviral drugs.

Tague, E.P.*, Dotson, H.L.*, Tunney, S.N.*, Sloas, D.C., Ngo, J.T.

Nature Methods, 2018, 15(7):519-522.  

PubMed  Publisher 
Plasmids on AddGene

Commentary in Nature Methods 
'Author File' in Nature Methods 

Coverage in Futurity

Click-electron microscopy for imaging metabolically tagged nonprotein biomolecules.

Ngo, J.T., Adams, S.R., Deerinck, T.J., Boassa, D., Rodriguez-Rivera, F., Palida, S.K., Bertozzi, C.R., Ellisman, M.H., Tsien, R.Y. 

Nature Chemical Biology, 2016, 12(6):459-65.  

PubMed  Publisher

"Greatest Hits" in Nature Chemical Biology 
Feature in Cell Chemical Biology 
Feature in Angewandte Chemie​

PKMζ, But Not PKCλ, Is Rapidly Synthesized and Degraded at the Neuronal Synapse.

Palida, S.F., Butko, M.T., Ngo, J.T., Mackey, M.R., Gross, L.A., Ellisman, M.H., Tsien, R.Y.

Journal of Neuroscience, 2015, 35(20):7736-7749.

PubMed

Cell-specific proteomic analysis in Caenorhabditis elegans.

Yuet, K.P., Doma, M.K., Ngo, J.T., Sweredoski, M.J., Graham, R.L., Moradian, A., Hess, S., Schuman, E.M., Sternberg, P.W., Tirrell, D.A.

Proc. Natl. Acad. Sci. USA. 2015, 112(9):2705-2710.

PubMed

Identification of Secreted Bacterial Proteins by Non-Canonical Amino Acid Tagging.

Mahdavi, A., Szychowski, J., Ngo, J.T., Sweredoski, M.J., Graham, R.L.J., Hess, D., Schneewind, O., Mazmanian, S.K., Tirrell, D.A.

Proc. Natl. Acad. Sci. USA. 2014, 111(1):433-438.

PubMed

Mutant Methionyl-tRNA Synthetase from Bacteria Enables Site-Selective N-terminal Labeling of Proteins Expressed in Mammalian Cells.

Ngo, J.T., Schuman, E.M., Tirrell, D.A.

Proc. Natl. Acad. Sci. USA. 2013, 110(13):4992-4997

PubMed

State-selective metabolic labeling of cellular proteins.

Ngo, J.T., Babin, B.M., Champion, J.A., Schuman, E.M., Tirrell, D.A.

ACS Chemical Biology, 2012, 7(8):1326-1330.
PubMed

Noncanonical amino acids in the interrogation of cellular protein synthesis.

Ngo, J.T., Tirrell, D.A.

Accounts of Chemical Research, 2011, 44(9):677-685.

PubMed

Cleavable biotin probes for labeling of biomolecules via azide-alkyne cycloaddition.

Szychowski, J., Mahdavi, A., Hodas, J.J., Bagert, J.D., Ngo, J.T., Landgraf, P., Dieterich D.C., Schuman, E.M., Tirrell, D.A. 

J. Am. Chem. Soc. 2010, 132(51):18351-18660.

PubMed

in situ visualization and dynamics of newly synthesized proteins in rat hippocampal neurons.

Dieterich, D.C., Hodas, J.J., Gouzer, G., Shadrin, I.Y., Ngo, J.T., Triller, A., Tirrell, D.A., Schuman, E.M. 

Nature Neuroscience, 2010, 13(7):897-905.

PubMed

Cell-selective metabolic labeling of proteins.

Ngo, J.T., Champion, J.A., Mahdavi, A., Tanrikulu, I.C., Beatty, K.E., Connor, R.E., Yoo, T.H., Dieterich, D.C., Schuman, E.M., Tirrell, D.A.

Nature Chemical Biology, 2009, 5(10):715-717.

PubMed

Unnatural amino acid incorporation into virus-like particles.

Strable, E., Prasuhn, D.E. Jr., Udit, A.K., Brown, S., Link, A.J., Ngo, J.T., Lander, G., Quispe, J., Potter, C.S., Carragher, B., Tirrell, D.A., Finn, M.G.
Bioconjugate Chemistry, 2008, 19(4):866-75.

PubMed