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Fe-TAMLs as a new class of small molecule peroxidase probes for correlated light and electron microscopy

Adams, S.R., Mackey, M.R., Ramachandra, R., Deerinck, T.J., Castillon, G.A., Phan, S., Hu, J., Boassa, D., Ngo, J.T., Ellisman, M.H.


Controlled protein activities with viral proteases, antiviral peptides, and antiviral drugs
Tague, E.P., McMahan, J.B., Tague, N., Dunlop, M.B., Ngo, J.T.

In press, ACS Chemical Biology

Tension-Tuned SynNotch Receptors for Synthetic Mechanotransduction and Intercellular Force Detection
Sloas, D.C., Tran, J.C., Marzilli, A.M., Ngo, J.T. 

Nature Biotechnology2023, 41: 1287–1295.

Feature in Nature Methods 

Plasmids on AddGene

SNAP-tag and HaloTag fused proteins for HaSX8-inducible control over synthetic biological functions in engineered mammalian cells.

Dotson, H.L., Ngo, J.T. 
Under Review.


A Genetically Encodable and Chemically Disruptable System for Synthetic Post-Translational Modification Dependent Signaling.

McMahan, J.B., Marzilli, A.M., Tran, J.C., Kuffner, C.J., Ngo, J.T. 

In Revision.


High-performance multiplex drug-gated CAR circuits.

Li, H,S., Wong, N.M., Tague, E.P., Ngo, J.T., Khalil, A.S., Wong, W.W. 

Cancer Cell


Precision control of intrabodies in live cells. (News and Views)

Marzilli, A.M., McMahan, J.B., Ngo, J.T.

Nature Methods, 2020, 17(3):259–260.


Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy.

Boassa, D., Lemieux, S.P., Lev-Ram, V., Hu, J., Xiong, Q., Phan, S., Mackey, M., Ramachandra, R., Peace, R.E., Adams, S.R., Ellisman, M.H., Ngo, J.T.

Cell Chemical Biology, 2019, 26(10):1407-1416.e5.  

PubMed  Publisher

Commentary in Nature Methods  

Plasmids on AddGene 

Plasmid #136601 is our reccomended option. All worked great, but Plasmid 136601 gave optimal cell to cell coexpression levels during DNA transfection with plasmid and also following LV transduction.

Chemogenetic control of gene expression and cell signaling with antiviral drugs.

Tague, E.P.*, Dotson, H.L.*, Tunney, S.N.*, Sloas, D.C., Ngo, J.T.

Nature Methods, 2018, 15(7):519-522.  

PubMed  Publisher 
Plasmids on AddGene

Commentary in Nature Methods 
'Author File' in Nature Methods 

Coverage in Futurity

Click-electron microscopy for imaging metabolically tagged nonprotein biomolecules.

Ngo, J.T., Adams, S.R., Deerinck, T.J., Boassa, D., Rodriguez-Rivera, F., Palida, S.K., Bertozzi, C.R., Ellisman, M.H., Tsien, R.Y. 

Nature Chemical Biology, 2016, 12(6):459-65.  

PubMed  Publisher

"Greatest Hits" in Nature Chemical Biology 
Feature in Cell Chemical Biology 
Feature in Angewandte Chemie​

PKMζ, But Not PKCλ, Is Rapidly Synthesized and Degraded at the Neuronal Synapse.

Palida, S.F., Butko, M.T., Ngo, J.T., Mackey, M.R., Gross, L.A., Ellisman, M.H., Tsien, R.Y.

Journal of Neuroscience, 2015, 35(20):7736-7749.


Cell-specific proteomic analysis in Caenorhabditis elegans.

Yuet, K.P., Doma, M.K., Ngo, J.T., Sweredoski, M.J., Graham, R.L., Moradian, A., Hess, S., Schuman, E.M., Sternberg, P.W., Tirrell, D.A.

Proc. Natl. Acad. Sci. USA. 2015, 112(9):2705-2710.


Identification of Secreted Bacterial Proteins by Non-Canonical Amino Acid Tagging.

Mahdavi, A., Szychowski, J., Ngo, J.T., Sweredoski, M.J., Graham, R.L.J., Hess, D., Schneewind, O., Mazmanian, S.K., Tirrell, D.A.

Proc. Natl. Acad. Sci. USA. 2014, 111(1):433-438.


Mutant Methionyl-tRNA Synthetase from Bacteria Enables Site-Selective N-terminal Labeling of Proteins Expressed in Mammalian Cells.

Ngo, J.T., Schuman, E.M., Tirrell, D.A.

Proc. Natl. Acad. Sci. USA. 2013, 110(13):4992-4997


State-selective metabolic labeling of cellular proteins.

Ngo, J.T., Babin, B.M., Champion, J.A., Schuman, E.M., Tirrell, D.A.

ACS Chemical Biology, 2012, 7(8):1326-1330.

Noncanonical amino acids in the interrogation of cellular protein synthesis.

Ngo, J.T., Tirrell, D.A.

Accounts of Chemical Research, 2011, 44(9):677-685.


Cleavable biotin probes for labeling of biomolecules via azide-alkyne cycloaddition.

Szychowski, J., Mahdavi, A., Hodas, J.J., Bagert, J.D., Ngo, J.T., Landgraf, P., Dieterich D.C., Schuman, E.M., Tirrell, D.A. 

J. Am. Chem. Soc. 2010, 132(51):18351-18660.


in situ visualization and dynamics of newly synthesized proteins in rat hippocampal neurons.

Dieterich, D.C., Hodas, J.J., Gouzer, G., Shadrin, I.Y., Ngo, J.T., Triller, A., Tirrell, D.A., Schuman, E.M. 

Nature Neuroscience2010, 13(7):897-905.


Cell-selective metabolic labeling of proteins.

Ngo, J.T., Champion, J.A., Mahdavi, A., Tanrikulu, I.C., Beatty, K.E., Connor, R.E., Yoo, T.H., Dieterich, D.C., Schuman, E.M., Tirrell, D.A.

Nature Chemical Biology, 2009, 5(10):715-717.


Unnatural amino acid incorporation into virus-like particles.

Strable, E., Prasuhn, D.E. Jr., Udit, A.K., Brown, S., Link, A.J., Ngo, J.T., Lander, G., Quispe, J., Potter, C.S., Carragher, B., Tirrell, D.A., Finn, M.G.
Bioconjugate Chemistry, 2008, 19(4):866-75.


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