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PEOPLE

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John T. Ngo, Ph.D.
Associate Professor of Biomedical Engineering

My students and I develop chemical and biophysical tools to probe, manipulate, and re-program how cells communicate. We then leverage these tools to gain insight into natural biology and overcome roadblocks in developing cell therapies and tissue-engineered systems. Our current efforts are focused on understanding contact-dependent cell signaling mechanisms, including Notch signaling and synaptic transmission, and their downstream outcomes. I was born in San Jose, California, and was the first in my family to go to college. I received a Ph.D. from Caltech in 2012 and was a post-doc at UC San Diego until 2015. In 2024, I was co-chair of the International Mammalian Synthetic Biology Workshop (mSBW). Students from the lab have received several awards and honors, including the BME Thesis of the Year Award and the College of Engineering's Earle and Mildred Bailey Memorial Award, among others.

IMG_0926.jpg
John T. Ngo, Ph.D.
Associate Professor of Biomedical Engineering

My students and I develop chemical and biophysical tools to probe, manipulate, and re-program how cells communicate. We then leverage these tools to gain insight into natural biology and overcome roadblocks in developing cell therapies and tissue-engineered systems. Our current efforts are focused on understanding contact-dependent cell signaling mechanisms, including Notch signaling and synaptic transmission, and their downstream outcomes. I was born in San Jose, California, and was the first in my family to go to college. I received a Ph.D. from Caltech in 2012 and was a post-doc at UC San Diego until 2015. In 2024, I Co-Chair of the International Mammalian Synthetic Biology Workshop (mSBW) in Boston, MA. Students from the lab have received several awards and honors, including the BME Thesis of the Year Award and the College of Engineering's Earle and Mildred Bailey Memorial Award, among others.

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Alex Marzilli, PhD

Post-Doctoral Fellow, Biomedical Engineering

Alex’s research is focused on developing tools to study and manipulate mRNA in living cells. Alex’s work uses a combination of protein and nucleic acid engineering to create novel post-transcriptional mRNA circuits that are responsive to a variety of biological ques, including small-molecule drug, light, and intracellular antigen levels.

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Jeremy Tran

Graduate Student, Biomedical Engineering

Jeremy's research is focused on developing synthetic Notch (SynNotch) systems to program mammalian cells to sense and interpret signals in their environment, such as mechanical force, ECM composition, and pericellular proteolysis. 

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Chris Kuffner,

Graduate Student, Biomedical Engineering

Chris uses genetic engineering to incorporate new functionality into RNA-protein interaction systems. He is currently applying these systems for high-contrast real-time imaging of single RNA molecules in live cells, where the system's engineered functionality has uncovered new insight into the spatiotemporal dynamics of post-transcriptional phenomena.

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Quan Le,

Graduate Student, Biomedical Engineering

Quan's research involves engineering drug-responsive nanobodies for controlling protein activities and signaling pathways, as well as nanobody-based tools for targeted protein degradation and visualization of translational activities in live cells.

Alumni
D. Christopher Sloas (Ph.D., 2020), BME Thesis of the Year Award 2020, Scientist, Carisma Therapeutics, Philadelphia, PA

Elliot P. Tague (Ph.D., 2020), George Church lab, Boston, MA
Krishna Harpanahalli (B.S., 2019, M.S., 2021), Moderna Therapeutics, Cambridge, MA
Remy E. Peace (Ph.D. 2021), Senior Associate Consultant, Clarion, Boston, MA
Jeffrey B. McMahan (Ph.D., 2021), Mammalian Synthetic Biologist, Asimov, Cambridge, MA
Deema Abdel-Meguid (B.S., 2021), Earle and Mildred Bailey Memorial Award, Graduate Student, Columbia University, New York, NY
Shannon Tunney (Ph.D., 2021), Scientist, aiGene, Denver, CO
Hannah L. Dotson (Ph.D., 2021), Senior Scientist, AddGene, Watertown, MA
Ezira Yimer Yolle (BS, 2019), Graduate Student, Boston University, Boston, MA
Jack Cardini (MS, 2024), Boston, MA
Rose Keating (MS, 2023), Draper, Cambridge, MA
Zach Silfen (MS, 2023), Boston University, Boston, MA

 
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